To study the effects of YSYT on the hematopoiesis of bone marrow cell, proliferation of splenic cell, to administration with cyclophosphamide(100㎎/㎏) in C57BL/6 mice, and on the gene expressing thrombopoietin (TPO), stem cell factor (SCF) and interleukin-3 of bone marrow cell, interleukin-10 (IL-10), interferon-γ (INF-γ) of splenic cell, active NOs change of macrophage in continuous medication YSYT, detected in serial sections using the RT-PCR technique. Splenic cells were analyzed using a flow cytometer. Two-color staining was performed with FITC-laveled CD4+, CD8+ and CD19+ for leucocytes and concluded as follows.
1. There´s no cytotoxicity of L+14 fibroblast cell from YSYT extract.
2. After the mice with leucopenia and thrombocytopenia CTX-induced were controled with oral administration with YSYT extract solution for 10 days. The number of WBC, RBC, platelet was increased to YSYT treatment group, compared with CTX control group.
3. After the mice with leucopenia and thrombocytopenia CTX-induced were controled with oral administration with YSYT extract solution for 10 days. Cell proliferation was significantly increased to YSYT treatment group, compared with CTX control group.
4. After the mice with leucopenia and thrombocytopenia CTX-induced was isolated bone marrow cells (BMC). Then, we found the increase of TPO, SCF, IL-3 gene expressions in BMCs with YSYT treatment group, compared with CTX control group.
5. After the mice with leucopenia and thrombocytopenia CTX-induced were controled with oral administration with YSYT extract solution for 10 days. Hemagglutinin titer was not significantly increased to YSYT treatment group, compared with CTX control group.
6. After the mice with leucopenia and thrombocytopenia CTX-induced were controled with oral administration with YSYT extract solution for 10 days. The number of CD4+, CD8+, CD19+ activated cells and surface-receptor expression were increased compared with control group.
7. After the mice with oral administration with YSYT extract solution for 10 days. Then, we found the increased of TPO, SCF gene expressions in BMCs with YSYT treatment group, compared with not-treatment group.
8. After the mice with oral administration with YSYT extract solution for 10 days. Then, we found the increased of IFN-γ gene expressions and decreased of IL-10 gene expression in splenic cells with YSYT treatment group, compared with not-treatment group.
9. After the mice with oral administration with YSYT extract solution for 10 days. Then, we found significantly increased of NOs Ⅱ gene expressions and NO production in macrophages with YSYT treatment group, compared with not-treatment group.
10. After the mice with oral administration with YSYT extract solution for 10 days. Then, we found significantly increased of the number of survival mice with EMC virus infection was long survived with YSYT treatment group, compared with not-treatment control group.
This study shows that YSYT has ameliorated to leucopenia and thrombocytopenia CTX-induced by oral admininstration with YSYT extract solution. From this study, YSYT is thought to be important in hematopoiesis and the immune response in mice.
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